of the Departament of Immunology, Rheumatology and Allergy
92 - 213 Łódź, ul. Pomorska 251
Tel. +48 42 675 73 20, 678 15 36, Fax +48 42 678 22 92
Head: dr n. med. Barbara Majkowska - Wojciechowska

Central Teaching Hospital 
Medical University of Łódź, Poland

Aeroallergen Monitoring Centre "AMoC" has started in 1995.

Main research topics: indoor and outdoor allergen studies
1. Pollen and mould spores counts analysis.
2. Detection of house dust allergens.
3. Measurement of fabrics permeability to house dust and Der p 1 allergen; determination of allergen accumulation properties of the fabrics.
4. Epidemiological Investigations of allergies and asthma in children in relation to environmental factors.

1. Research on outdoor allergena
Aeroallergens measurement consists mainly of:
1.1. 1.1. Analysis of pollen count in downtown Łódź area

The tape with sticky silicon oil is placed in the Lanzoni apparatus every week during the whole season, exposed for seven days of weekPollen count analyses in Lodz have been conducted from 2003 since it was difficult to imagine modern allergology without referring to data obtained from pollen count monitoring in the atmosphere. The availability of pollen and aeroallergen count results helps in the diagnostics of pollinosis, prevention of its symptoms, enables establishing the safest immunotherapy protocols, including the choice of best possible timing and dosing and also helps in epidemiological research
The studies were conducted using a volumetric method with the Lanzoni apparatus. The measurements (35 weeks)- from 1 February to 30 September. The tape with sticky silicon oil placed in the Lanzoni apparatus was fitted once a week during the whole season, exposed for 7 days of the week and cut into sections corresponding to each day. After dyeing the tape, 20 pollen taxons and two mould taxons of different systemic value prevailing in the fall and/or relevant from the clinical point of view were determined. All the preparations were analyzed by one person in agrement with international methods.

Topographic parameters of measurement point:
The measurement point was situated at the distance of about 30 km from geometric centre of Poland, in the centre of Łódź (51º46’17,5”N; 19º28’29,3”E), which is situated in the mesoregion of the Łódź Hills situated about 150 m above the sea level.
Botanical parameters:
In the centre of Łódź, where the Lanzoni apparatus is situated, there are four parks and small squares with decorative plants, synantropic herbaceous plants and grass, which is mowed quite frequently. In the nearest neighbourhood (Uniwersytecka, Narutowicza, Wierzbowa and Jaracza Streets), the architecture is quite dense and consists of old tenement houses and modern buildings of the University of Łódź and the Medical University. There is a small park nearby, named after Stanisław Staszic, where numerous species of trees and bushes are grown, both domestic and foregin. In the proximity of the measurement point, there are some birches and Crimean time-trees along Narutowicza street, as well as some Italian poplars, sugar maples, a maiden – hair tree, ash-trees, decorative apple trees and some other species.

Presentation of the obtained results
WFor clinical and scientific purposes, it is essential to record and present on demand the measurements of pollen counts, particularly of Łódź agglomeration - important place in Central Poland.
The news and weather forecasts for the following week, including the data on pollen counts in relation to short-term and long-term weather forecast, are available at the beginning of every week on the local

Pollen count analysis in urban/rural area
The aim of the study was to compare the concentrations of pollen in urban and rural environments of Central Poland area.
Pollen counts were obtained weekly, using volumetric method with two Lanzoni pollen traps in Łódź and in Mierzyce since March till October in 2003 and 2004. We found more than 90 pollen taxa in both regions. The study demonstrates that significant differences in pollen fall in rural and urban area do not explain differences in prevalence of pollen sensitization between rural and urban areas.

2. Analysis of house dust allergens concentration

Analysis of indoor allergens: Der p 1, Fel d 1 and Can d 1
Allergy to house dust allergens is a main source of sensitization and an important trigger of asthmatic symptoms in asthmatic patients in Poland. The relationship between allergen exposure and sensitization is complex and seems to be different for various allergens. As a part of study on environmental factors affecting allergy and asthma, dust samples were collected from dwellings of urban (Łódź) and rural (Słupia and Mierzyce) areas and Der p 1, Fel d 1 and Can f 1. Allergen concentration in dust sample was detected by double-antibody ELISA assay. Der p 1 levels in homes in central Poland was similar but lower to previously reported in other European countries of the temperature climatic zone and was strongly related to the house characteristics. We also identified several factors related to indoor environment which have influence on allergen levels and should be considered while preventive measures are taken.

3. Measurment of fabrics

3.1. Measurement of fabrics permeability to house dust and Der p 1 allergen and mite allergen accumulation.

Using original methods, developed in our laboratory, we tested plenty of fabrics, manufactured with the use of various technologies. We were able to chose a single fabrics “Argo” manufactured by Instytut Inżynierii Materiałów Włókienniczych in Łódź, which demonstrated permeability to house dust and Der p 1 allergen below 1% of reference cotton fabric.

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Cooworkers of Aeroalerge Monitoring Centre
Prof. Dr hab. med. Marek L. Kowalski
Dr Barbara Majkowska-Wojciechowska
Mgr Zofia Balwierz
Mgr Marzanna Jarzębska
technik Beata Świerczewska
technik Katarzyna Lewiak
Krzysztof Wojciechowski